Two cytotypes of Kiefferulus tendipediformis (Goetghebuer, 1921) (Diptera, Chironomidae)

The material identified as Kiefferulus tendipediformis on the basis of the larval morphology consisted of two cytogenetically distinct species, which we called cytotype 1 (2n=8) (Bulgaria) and cytotype 2 (2n=6) (Poland). The karyotype and external morphology (larva, pupa and imago males) of Kiefferulus tendipediformis from pond on mine spoils in the Olkusz region with Zn-Pb ore deposits (Southern Poland) are described. The karyotype of cytotype 2 (2n =6) (Poland) consists of two metacentric chromosomes (AB and CD) and one submetacentric GEF. The chromosomes AB, CD of both cytotypes are very similar, the chromosome GEF is formed through tandem fusion of two acrocentric chromosomes of cytotype 1 (chromosomes EF and G). The external morphology of the species from Poland is compared with materials of the same species from Bulgaria and Austria. The larvae of Poland and Bulgaria are differed in a number of ultrastructure characters. Pupae and imagoes of K.tendipediformis from Poland are distinguished from those of Austria. The current taxonomic position of the K.tendipediformis in Europe is discussed.